ABSTRACT
The steadily increasing number of biological applications of cryoelectron microscopy of unstained vitrified specimens has made the selection of topics and representative examples difficult. As with negative staining (Chapter 5) the space available for applications together with their structural and biological interpretation is restricted and inevitably the selection will be incomplete. However, the interested reader should easily find relevant publications in the literature and initially the surveys by Dubochet et al. (1986) and Stewart (1991) should be useful. Thin film cryoelectron microscopy has been applied almost entirely to isolated systems, rather than intact cells and organelles. However, one example which parallels negative staining studies, is the application of high voltage cryoelectron microscopy to the study of platelet structure (O'Toole et al., 1993).
