ABSTRACT

Optimal embryo culture conditions are essential for embryo development and survival.

Monitoring and maintaining the temperature, pH, gas composition, VOCs, culture media, and humidity is necessary.

Regular quality control checks contribute to successful IVF outcomes by ensuring a stable culture environment.

Monitoring embryo development helps identify issues and optimises embryo selection for transfer.

Cleavage-stage embryo evaluation involves assessing cell division, cell size, and fragmentation.

The evaluation of blastocyst development encompasses observing and analysing the inner cell mass, trophectoderm, and blastocoel cavity.

Timelapse imaging can provide additional insights into embryo development and aids in selecting high-quality embryos.

Troubleshooting common embryo culture issues, such as slow or arrested development, fragmentation, and multinucleation, is essential for improving IVF success rates.

Regular staff training, adherence to established protocols, and thorough documentation can help prevent and resolve issues during embryo culturing.