ABSTRACT

Cryopreservation involves preserving embryos, sperm, and oocytes at extremely low temperatures (–196 °C) using liquid nitrogen.

Quality control is essential to maintaining the viability of preserved gametes and embryos for successful IVF outcomes.

Proper labelling, storage, and handling of samples are crucial aspects of quality control.

Cryopreservation in ART has enhanced fertility treatments but introduced challenges to fertility clinics like accumulating stored embryos, financial burdens, logistical complexities, and ethical dilemmas regarding the disposal of potential life forms.

Performing routine maintenance on cryopreservation equipment is advisable to maintain reliable and consistent performance.

Resolving problems related to the loss, contamination, or damage of samples during freezing and thawing is paramount.

Staff training, protocol adherence, and equipment maintenance can minimise cryopreservation problems.

Preventing embryos or gametes from falling into a liquid nitrogen storage tank involves mitigating human error, equipment failure, and inadequate protocols.

Implementing secondary containment systems helps to prevent accidents.

Encouraging a culture of open communication and accountability improves transparency and overall safety in IVF labs.

It is recommended to periodically review and update protocols to ensure accuracy and alignment with the latest advancements in ART fields.

Components of an emergency response plan include staff training, clear communication channels, and established protocols for handling various scenarios.