ABSTRACT
This chapter briefly focuses on recent progress in, and long-term promise of, the use of CRISPR technology for introducing targeted modifications into host genomes with the goal of enhancing resistance against plant parasitic nematodes. Genome editing is a targeted and more exact form of mutation breeding. Genome editing is a process where an organism’s genetic code is changed. CRISPR/Cas-based tools were also effectively harnessed to generate stress-tolerance in species, model plant, and commercial crop varieties. Genetic modifications can be introduced without foreign gene integration, one of the significant advantages of CRISPR/Cas tools. Variously fuse cytidine or adenine deaminases to Cas proteins to enzymatically convert endogenous bases to introduce a very specific set of edits within a narrow window. Genome editing as an advanced molecular biology technique that can produce precisely targeted modifications in any crop.
