ABSTRACT
The main difficulty with culturing animal cell lines is that the cell membrane of animal cells is very thin and weak, so many difficulties crop up when culturing animal cell lines with the existing conventional bioreactors. For example, in the case of the continuous stirred tank reactor, a substantial amount of animal cells will be destroyed by the impinging fan blades and the resultant shear force generated inside the bioreactor. A generalized bioprocess model having five states and four inputs has been formulated. There are nine operating modes in which a bioprocess can be operated. First, an operating mode is selected for a particular process, and then genetic algorithms are applied to find optimal time trajectories of the states. A fermentation process can be explained as follows: "the microorganisms grow with the consumption of certain nutrients assuming that the environmental conditions are favourable". The chapter also presents an overview of the key concepts discussed in this book.
