ABSTRACT
This chapter briefly discusses the application, chemical properties and physical properties of benzidine. It presents detailed information on the analysis of aqueous and nonaqueous samples of benzidine. Benzidine is used in the past in the manufacture of dyes. It is a white crystalline solid, slightly soluble in cold water, and moderately soluble in hot water, alcohol and ether. During the analysis of aqueous and nonaqueous samples of benzidine, aqueous samples are extracted with chloroform. The chloroform extract is further extracted with 1 M H2SO4. The acid extract is buffered with 0.4 M sodium tribasic phosphate and then pH is adjusted between 6 and 7 using NaOH. The neutralized extract is then treated with chloroform. Then, benzidine is analyzed by high-performance liquid chromatography (HPLC) using an electrochemical detector.
