ABSTRACT
Low levels of antibodies to N. caninum have been reported in human sera. The significance of these findings is uncertain because neither parasite DNA nor the parasite has been confirmed in human tissues. Nevertheless, N. caninum has been successfully cultured in human cell lines such as MCF-7 human breast carcinoma, human brain microvascular endothelial cells, human trophoblast and uterine cervical, human foreskin fibroblasts, human astrocytoma cell line, human epithelial lung cells and also, the same level of growth was observed when human serum instead of fetal bovine serum was used for tissue culture. Transplacental neosporosis was demonstrated in Macaca mulatta in 2 experiments performed in California, USA.
