ABSTRACT

Metabolic engineering strategies for the production of a variety of biofuels and biochemicals by microorganisms are explained. The organic acid fermentations such as pyruvate, lactate, citric acid, malic acid, fumaric acid, and succinate fermentations are explained with pathway modification of Escherichia coli, Corynebacterium glutamicum, and others. As for biofuels production, hydrogen, ethanol, 1-butanol, 1-propanol, isobutanol, and isopropanol fermentations are briefly explained for engineered Saccharomyces cerevisiae, Zymomonas sp., E. coli, and Corynebacterium sp., etc. Metabolic engineering of E. coli and Corynebacterium sp. for shikimic acid and amino acids fermentations is also explained. The production of isoprenoids by microorganisms is also explained. The production of diols such as butandiol and propandiol from glycerol is also explained focusing on the use of engineered E. coli.