ABSTRACT
The bacterial population plays an important role in the induction and appearance of disease symptoms. Even a single bacterium can initiate the infection but the time taken is relatively longer. More bacterial population means quicker symptoms development. Intercellular fluid (IF) provide a perfect nutrition for the growth and multiplication of bacterial plant pathogen. Similarly, it can be used for isolation of bacterial metabolites like enzymes and toxins of bacterial plant pathogens. The method to obtain IF from leaves is the same as was used by Klement. Pluck the leaves of the host plant and washed thoroughly with distilled water. 25 ml of IF is sterilized by filtration through bacteriological sintered glass filter for further use to study the bacterial growth, enzyme production, or toxin production by the bacterial plant pathogen.
