ABSTRACT
DNA sequences are usually inserted into a cloning vector for manipulation. When sequencing, these constructs frequently produce raw sequences that include segments derived from a vector. If the vector part of the raw sequence is not removed, the finished sequence will be contaminated, spoiling further analysis. There are multiple sources of DNA contamination, like transposons, insertion sequences, organisms infecting our samples, and other organisms used in the same laboratory (e.g., cross contamination from dirty equipment).
