ABSTRACT

A researcher needs to design a DNA sequence based on a coding sequence. This sequence codes for a polypeptide that the researcher is interested in. The new sequence has to code for the same polypeptide, so all nucleotide changes in the new sequence must be “silent mutations.” A silent mutation is one that does not result in a change to the amino acid sequence of the resulting protein. This is done by taking advantage of the DNA code redundancy. For example AAG codes for “Lysine” (K), as AAA, so changing G for A doesn’t change the resulting polypeptide.