ABSTRACT
The greatest progress towards the development of nutrition medium for plant cells grown in culture took place in 1960s and 1970s. The basic nutritional requirements of cultured plant cells are very similar to those utilized by plants. However, the nutritional composition varies according to the cells, tissues, organs and protoplasts and also with respect to particular plant species. Different types of culture have unique requirements of one or more nutritional components. A nutrient medium is defined by its mineral salt composition, carbon source, vitamins, growth regulators and other organic supplements which include organic nitrogen, acids and complex substances. The mineral composition of culture medium is defined precisely by the equilibrium of the concentrations of different ions in a solution. To choose a mineral composition and then compare their different ionic balances, one uses ionic concentrations expressed in milliequivalents per litre.
