ABSTRACT
Fluorescence microscopy and spectroscopy are versatile tools which have becom e standard techniques in many branches of physics, chemistry, and biology. One of the m ost advantageous qualities of these methods is that the measured photophysical properties o f the investigated entities (e.g., dye molecules or nanocrystals) allow a deep insight into other fundamental physical or chemical processes within themselves or their direct environment. Thus fluorescent molecules or other particles can be utilized as probes which are reliable, comparably cheap, and relatively easily accessible by experiment. However, the interpretation of the measured data is in many cases not trivial.
