ABSTRACT
Time-resolved uorescence measurement has wide applications in medical diagnostics and molecular imaging. It is a technique in which the decaying emission intensity of a uorescence molecule, or uorophore, is recorded with a high temporal resolution. A time constant, also known as the uorescence lifetime, is subsequently extracted.1 This lifetime reects the surrounding chemical composition of the uorophore and their interaction. For example, Förster resonance energy transfer (FRET),2 quenching, and molecular rotation3 are phenomena that uorescence lifetime measurements can examine effectively.
