ABSTRACT
I don’t suppose you happen to know Why the sky is blue?… Look for yourself. You can see it’s true.
Light microscopy, invented over 300 years ago, has revolutionized our understanding of biological processes. In its modern form, it involves much more than just the magnification of images in biological samples. There are invaluable techniques that have been developed to increase the image contrast. Fluorescence microscopy in particular is a very useful tool for probing biological processes. It results in high signal-to-noise ratios (SNRs) for determining the localization of biological molecules tagged with a fluorescent dye but does so in a way that is relatively noninvasive. This minimal perturbation to the native biology makes it a tool of choice in many biophysical investigations.
