ABSTRACT
Applications for liquid chromatography–nuclear magnetic resonance (LC–NMR) in the pharmaceutical sciences include, but are not limited to, isolation and characterization of drug impurities resulting from drug-stability tests, synthetic intermediates, drug production, and formulation reactivity. LC–NMR has been successfully used to isolate and identify structures ranging from natural products to applications in drug metabolism and the analysis biofluids This chapter describes applications that combine LC with NMR spectroscopy to address problems in key areas of interest in both the pharmaceutical industry and academia. These applications cover LC–NMR applications of degradation products, impurities, trace analysis, analysis of mixtures, formulation reactivity, tautomer kinetics, unstable products, metabolites, natural products, and capillary isotachophoresis (cITP) isolates.
