ABSTRACT
Nuclear magnetic resonance (NMR) can be used as a quantitative technology to evaluate a compound’s purity, concentration, and quantity of material. The method termed quantitative NMR (qNMR) spectroscopy can provide an accurate and precise method that can complement and/or corroborate the HPLC method. The proportional relationship of signal intensity to the number of nuclei of a particular spectral peak enables a precise measurement of the amount of a structurally characterized compound. With qNMR, no expensive chemical reference substances are necessary and structural information may be determined from the same sample. Use of multiple nuclei (1H, 19F, and 31P) is also possible, which expands the range and versatility of this method. The qNMR assay is a robust method having high reproducibility. When combined with ERETIC2/PULCON, qNMR provides a powerful means of determining purity, concentration, and mass of a compound. Examples include quantifying isolated metabolites, natural products, and complex compound mixtures.
