ABSTRACT
Contents 8.1 Overview ............................................................................................................................... 97
8.1.1 DNA and RNA Chemistry ...................................................................................... 97 8.2 Protoco1s .............................................................................................................................. 100
8.2.1 Isolation of Tomato DNA for DNA Amplification by PCR ................................ 100 8.2.2 DNA Extraction Procedure for RFLP Analysis Beginning with
Young Leaves ........................................................................................................ 102 8.2.3 Extraction of Bulk RNA ....................................................................................... 103 8.2.4 Isolation of Poly A-RNA from Bulk RNA ........................................................... 104 8.2.5 Estimation of DNA Fragment Size and Quantitation .......................................... 1 04
References ...................................................................................................................................... 1 06
Nucleic acids (DNA and RNA) are composed of covalently linked nucleotides that consist of nitrogenous bases (purine and pyrimidine), a pentose (deoxyribose or ribose), and a phosphate group. The bases are attached to deoxyribose in DNA or ribose in DNA through carbon 1 of the monosaccharide. In contrast, the phosphate groups are linked to the monosaccharide at the C-51 and C-3 1 positions (Figure 8.1).
