ABSTRACT
References ...................................................................................................................................... 151
Abbreviations
A de Ado AMP CPM DEAE DHZ DHZR GC/MS HPLC lAC iP fl2_jpp [9R]iP [9R-5'P]iP [7G]iP Kinetm OGDHZ OGDHZR OGZ OGZR oxz z [7G]Z [9G]Z ZR [9R-5'P]Z
Adenine Adenosme Adenosine-5' -monophosphate Counts per minute Diethylaminoethyl cellulose anion exchanger Dihydrozeatin Dihydrozeatin riboside Gas chromatography/mass spectrometry High-performance liquid chromatography lmmunoaffinity column N6(/l2-Isopentenyl)adenine !l2-Isopentyl pyrophosphate N6(/l2-lsopenteny !)adenosine N6(/l2-Isopenteny !)adenosine-5' -monophosphate N6(/l2-lsopentenyl adenine-7 -glucoside) 6-Furfury laminopurine 0-Glucosyl dihydrozeatin 0-Glucosyl dihydrozeatin riboside 0-Glucosyl zeatin 0-Glucosyl zeatin riboside 0-Xylose zeatin trans-Zeatin, 6-( 4-hydroxy-3-methyl but -trans-2-enylamino )purine trans-Zeatin-? -glucoside trans-Zeatin-9-glucosJde trans-Zeatin riboside trans-Zeatin riboside-5'-monophosphate
Methods for analysis of cytokinin content, biosynthesis, metabolism, and catabolism as they relate to plant responses is the focus of this chapter. Recently, several excellent reviews of the physiology and biochemistry of cytokinin in plants have appeared. The reader is recommended to them for
additional information. 1-3 Plant cellular responses to cytokinin are complex. Although defined by the ability to promote cell division,4 cytokinin can also promote cell expansion and chloroplast development.5 Cytokinin appears to regulate certain events in plant organ development including shoot bud differentiation,6 bud break,? senescence,5 leaf expansion, and reproductive development.8 Cytokinin almost always acts synergistically or antagonistically in association with other plant growth regulators.
