ABSTRACT

Contents 25.1 Introduction ......................................................................................................................... 310 25.2 Polysaccharidases ................................................................................................................ 310

25.2.1 Nelson-Somogyi .................................................................................................. .310 25.2.2 Dinitrosalicylic Acid (DNS) .................................................................................. 311 25.2.3 Viscosimetric ......................................................................................................... 311 25.2.4 Cellulase ................................................................................................................ 312 25.2.5 Hemicellulases ....................................................................................................... 313

25.2.5.1 Xylanase .............................................................................................. .313 25.2.5.2 Mannanase ............................................................................................ 315

25.2.6 Pectinase ................................................................................................................ 316 25.2.6.1 Pectin Methylesterase .......................................................................... 316 25.2.6.2 Polygalacturonase ................................................................................ .316 25.2.6.3 Pectin Lyase ......................................................................................... 316

25.2.7 Lytic Enzymes ....................................................................................................... 317 25.2.7.1 Chitinase ............................................................................................... 317 25.2.7.2 ~-1,3-Glucanase ................................................................................... 317 25.2.7.3 Lysozyme ............................................................................................. 317

25.2.8 Amylase ................................................................................................................ .317 25.2.9 Dextranase ............................................................................................................. 318

25.3 Glycosidases ........................................................................................................................ 318 25.4 Microplate Assay ................................................................................................................. 318 25.5 Screening Assays ................................................................................................................ .319