ABSTRACT

Introduction ........................................................................................................ 289

Materials and Methods ..................................................................................... 292

Results and Discussion ..................................................................................... 292

Conclusion .......................................................................................................... 298

Acknowledgments ............................................................................................. 299

References ........................................................................................................... 299

Cloudiness of turbid or opalescent apple juice is provided by particulate

material that remains in suspension (Genovese et al., 1997). This cloud has

beenmodeled to consist of negatively charged pectin wrapped around a core

of positively charged protein (Yamasaki et al., 1964; Endo, 1997). Particle

sizes above 0.5 mm are unstable and settle out (Beveridge, 2002). Below this

range, particles are colloidal in nature and follow Brownian motion. These

colloidal particles are retained in suspension as a result of their small size,

mutual charge repulsion, and the protective effect of pectin (Beveridge,

Pectin acts as a protective colloid in turbid juices, retarding the

coagulation and precipitation of small insoluble particles. Pectin slows

aggregation by coating the particles, preventing the close-enough approach

of one to another needed for the formation of hydrogen bonds or effective

dipole interactions. In addition, it may give particles a large enough

charge that significant electrostatic repulsion takes place between particles

(Van Buren, 1989). However, the colloidal mechanism of particle stabiliz-

ation in cloudy juices is still unclear.