ABSTRACT
Preparation of Medium: Add components, except Fraser supplement solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121°C. Cool to 45°–50°C. Aseptically add sterile Fraser supplement solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
