ABSTRACT
CoSO4 ..............................................................1.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add 4-hydroxybenzoic acid and nitrilotriacetic acid to approximately 600.0mL of distilled/deionized water. Adjust pH to 8.0 with concentrated NaOH. Add remaining components. Mix thoroughly. Readjust pH to 7.2. Bring volume to 1.0L with distilled/deionized water. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121°C.
