Yeast extract........................................................1.0g MgSO4·7H2O......................................................0.2g MnCl2·4H2O ..................................................20.0mg CaCl2................................................................1.2mg Glucose-uric acid solution .......................... 100.0mL

Glucose-Uric Acid Solution: Composition per 100.0mL: Glucose ...............................................................5.0g Uric acid..............................................................0.4g

Preparation of Glucose-Uric Acid Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.