ABSTRACT
Over the past decade, those engaged in the analysis of protein toxins generally focus on one or more attributes of the protein toxin or on the interaction of the toxin with the physiology of the target. Then, applying generally good and sophisticated scientific practice, highly specific conclusions are drawn regarding what was required for
2.1 Protein Toxins: Life Stages and Primary Focus ............................................. 31 2.1.1 Introduction and Definitions ............................................................... 31 2.1.2 Researching a Toxin Life Cycle .......................................................... 35
2.2 The Critical Role of Conformational Flexibility in the Toxin Life Cycle ...... 35 2.2.1 Studying Protein Conformational Changes ........................................ 35 2.2.2 Forces that Contribute to Protein Stability ......................................... 38
2.3 The Energetics of Protein Folding and Application to Protein Toxin Mode of Action .................................................................... 39 2.3.1 Folding of Soluble Proteins ................................................................. 39 2.3.2 Folding of Membrane Proteins ...........................................................40
