ABSTRACT

Introduction ............................................................................................................ 190 Background: Peripheral Gustatory System ............................................................ 191 Central Gustatory-Reward Neural Pathways ......................................................... 194

Taste Coding in the Central Nervous System ............................................. 195 Simultaneous Recordings of Multiple Single Neurons in Awake and

Behaving Rodents ....................................................................................... 195 Methods for Chronic Implantation of Multiple Microwires ....................... 197 Methods for Neural Ensemble Recordings in Awake and Behaving

Animals ............................................................................................ 198 Methods for Histological Conrmation of Electrode’s Tip Placement ......200

Neural Ensemble Recordings in Freely Licking Rodents ......................................200 Methods for Neural Ensemble Recordings in Freely Licking Rodents ......203

Neural Correlates of Preference, Hunger, and Satiety in Freely Licking Rodents ........................................................................................................205 Methods for Measuring Preference in Freely Licking Rodents .................205 Neural Ensemble Recordings in Taste-Reward Pathways ..........................207 Methods for Measuring Hunger and Satiety ...............................................209 Methods for Measuring or Manipulating Levels of Metabolic Factors