ABSTRACT
Overview ................................................................................................................ 216 12.1 Introduction ................................................................................................. 216 12.2 Historical Perspective on Noncoding RNA ................................................. 217 12.3 What Are miRNAs? .................................................................................... 217 12.4 Which Methods Are Available for miRNA Expression Profiling? ............. 218 12.5 Isolation and Fractionation of Small RNA .................................................. 218
12.5.1 Total RNA Extraction Using Phenol-Containing Reagents ............ 221 12.5.2 Elution of Small RNAs from Gel Slabs........................................... 221 12.5.3 Methods for miRNA Enrichment .................................................... 222
12.6 miRNA Expression Profiling Using Microarrays ....................................... 222 12.6.1 Experimental Designs for miRNA-Specific Microarray
Platforms.......................................................................................... 222 12.6.1.1 miRNA-Specific Microarray Platforms That Rely on
Tm Normalization of the Capture Probes .......................... 223 12.6.2 Alternative Principles for miRNA Profiling Using Microarrays ....224
