ABSTRACT
In interface development for liquid chromatography–mass spectrometry (LC–MS), the mobile phase was initially considered as a disturbing and restricting factor: it should be evaporated prior to introduction into the mass spectrometer. Subsequently, strategies were considered that actually take advantage of the presence of the mobile phase (cf. Ch. 3.3.3). The ability of the direct liquid introduction (DLI) interface to transfer highly labile compounds, e.g., vitamin B12 (Figure 4.7), to the gas phase and make them available to chemical ionization (CI) is believed to be strongly supported by the presence of the mobile phase. The analytes included in the desolvating droplets are subjected to in-beam or direct chemical ionization [1-2].
