ABSTRACT
Quantitation of drugs and their metabolites in biological matrices currently is one of the most important applications of LC–MS. This can be attributed to the greatly enhanced selectivity and reliability of the analysis, as compared to LC–UV. Selective reaction monitoring (SRM) in tandem mass spectrometry (MS–MS) has become the method-of-choice in quantitative bioanalysis. Ample examples are described in literature. Even a larger number of successful examples are hidden in the archives of pharmaceutical companies and contract research organizations.
