ABSTRACT
Chromatography is a physical separation method in which the components to be separated are selectively distributed between two immiscible phases: a mobile phase is flowing through a stationary phase bed. The technique is named after the mobile phase: gas chromatography (GC), liquid chromatography (LC), or supercritical fluid chromatography (SFC). The chromatographic process occurs as a result of repeated sorption/desorption steps during the movement of the analytes along the stationary phase. The separation is due to the differences in distribution coefficients of the individual analytes in the sample. Theoretical and practical aspects of LC have been covered in detail elsewhere [1-5].
