ABSTRACT
Introduction........................................................................................................... 110 Subjects and Methods........................................................................................... 110
Skeletal Muscle Biopsy Procedure........................................................... 111 Adipose Tissue Biopsy ............................................................................. 112
Blood Sampling and Leukocyte Isolation............................................................ 112 Tolerability Assessment ............................................................................ 112
RNA Extraction .................................................................................................... 113 Microarray Hybridization ......................................................................... 113
Microarray Statistical Analysis ............................................................................ 113 Correlations................................................................................................113 Normalization and Elimination of Outlier Data Points ........................... 113 Normalization among Samples within Each Tissue ................................ 114 Clustering Analysis................................................................................... 114
Results................................................................................................................... 114 Tolerability Biopsy Procedures ................................................................ 114 Biopsy Weight and RNA Yield ................................................................ 115
Evaluation of Gene Expression Profiles in Biopsy Tissues and White Blood Cells ......................................................................................... 116 Discussion ............................................................................................................. 120 References............................................................................................................. 122
Since the publication of the first microarray experiment in 1995 [1], this technology has rapidly evolved and has now become one of the major tools for global geneexpression profiling. The results of recent microarray studies have greatly added to our understanding of e.g., disease pathways [2-5], mechanisms of drug action [6], and tumor classification [7,8] underlining the increasingly important role of geneexpression profiling in biomedicine.
