ABSTRACT

Introduction............................................................................................................262 Ribo-SPIA™: A Novel Method for Global Isothermal Linear Amplification of mRNA.............................................................................263 Ribo-SPIA Protocol for Rapid Robust Amplification of Total mRNA in Small Total RNA Samples ..........................................................265 Accuracy, Reproducibility, and Linearity of Ribo-SPIA Assessed by qPCR.................................................................................................267 Expression Analysis Using the Ribo-SPIA Amplification and GeneChip Arrays ............................................................................................269