ABSTRACT
Split-Ubiquitin Membrane Yeast Two-Hybrid System................... 137 8.5 Conclusion ..................................................................................................141 Acknowledgments ................................................................................................142 References ............................................................................................................142
During the last decades it has become clear that membrane proteins play a dominant role in many cellular functions and are involved in cardiovascular, metabolic, neurological, psychiatric and oncological diseases. The sequencing of the human genome revealed that approximately 30% of human genes code for membrane proteins.1,2 From the viewpoint of pharmacology, membrane proteins are the most important protein family. Many pharmaceuticals mediate intracellular effects by targeting membrane proteins and, as a result, the study of membrane proteins has become one of the most active fields in biochemical and pharmacological research.
