ABSTRACT
Dehalogenase ............................................................................... 738
30.7 Conclusion and Prognosis........................................................................................739
References .......................................................................................................................... 739
718 Biocatalysis in the Pharmaceutical and Biotechnology Industries
The advent of advanced enzyme evolution technologies promises to be the breakthrough
technology that will transform biocatalysis into a robust, economically attractive methodology
for themanufacture of complex chiral molecules. Formany decades, enzymes have been touted
as promising catalysts in chemical applications, yet the number of commercial biocatalytic
processes has grown only slowly. Recently developed enzyme evolution technologies allow
for the rapid development of tailor-made catalysts for commercial processes. This chapter
discusses the reasons why such technologies were needed, provides an overview of the different
tools that are available and the rationale that guides enzyme evolution, and summarizes
recent successes.
