ABSTRACT

Recipient Cytoplasts ........................................................................116 8.3 Effect of Donor Nucleus on Nuclear Transfer ............................................117

8.3.1 Cell Synchronization of Domestic and Nondomestic Cat Cells.....118 8.3.2 Effect of Cell Dissociation on the Cell Cycle and Cell Integrity...120 8.3.3 Distinguishing G0 and G1 Cell Populations ...................................121 8.3.4 Effect of Donor Nucleus Type and Cell Cycle Synchronization

Method on Embryo Reconstruction and In Vitro Development......127 8.3.5 Chromosomal Stability of Cat Somatic Cells and Derived

Cloned Embryos...............................................................................129 8.4 Activation of Domestic Cat Oocytes ...........................................................131