ABSTRACT
Since 1997, births of cloned animals following nuclear transplantation (NT) with somatic cells have been reported for several mammalian species (see other chapters in this book). Two independent protocols have been applied for producing reconstructed zygotes:
• A single donor cell is fused with an enucleated oocyte and the couplet is immediately activated (cell fusion method, e.g., in sheep [1] and cattle [2]).
