ABSTRACT
HPLC columns used for the analysis of water-soluble vitamins are of the same type as those used in fat-soluble vitamin assays (see Chapter 20, Section 20.4.3).
21.1.2 Chromatographic Modes
The choice of chromatographic mode for quantitative analysis depends on the extraction and cleanup procedures employed and the vitamins required to be measured. Chromatographic modes used in water-soluble vitamin assays include normal-and reversed-phase chromatography (see Section 20.4.4), ion exchange chromatography, ion exclusion chromatography, and reversed-phase ion-pair (ion interaction) chromatography.
