ABSTRACT

CONTENTS 8.1 Introduction ............................................................................................... 191 8.2 Molecular-Based Identification Methods.............................................. 193

8.2.1 Isolation of DNA.......................................................................... 193 8.2.2 Primers for Identification of Probiotic Bacteria ...................... 194 8.2.3 PCR-Based Identification Methods ........................................... 198 8.2.4 Restriction Enzyme Analysis-Based Identification Methods .. 200

8.3 Future Directions ...................................................................................... 201 8.4 Conclusion ................................................................................................. 202 References............................................................................................................ 202

The word probiotic is derived from the Greek meaning “for life”. Probiotics are mainly consumed by eating foods or supplements that contain a monoor mixed culture of live bacteria. The microorganisms exist in a viable state in foods like dairy products or as lyophilized forms in supplements. The beneficial effects attributed to the consumption of probiotic bacteria include improving intestinal tract health, enhancing the immune system, synthesizing and enhancing the bioavailability of nutrients, reducing symptoms of lactose intolerance, decreasing the prevalence of allergy in susceptible individuals, and reducing the risk of chronic disorders like ulcerative colitis and colorectal cancers (1-9).