ABSTRACT
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There are many well-documented approaches to purifying antibody from a variety of sources (for reviews, see References 1 and 2). The method of choice will depend upon the antibody source, time available, cost considerations, and final use of the antibody. This chapter is organized to provide the reader with options that involve single steps for purifying IgG and IgM that will save time but will be less cost effective. Alternative two-step methods are also provided that will cost less in terms of supplies but will require more time to complete. In each section, a brief paragraph provides information on the advantages and disadvantages of each method and gives examples of when each method might be best utilized.
