ABSTRACT
The full characterization of hyaluronic acid (HA) in the early years of its discovery was a rather difficult and tedious task, because of the lack of sophisticated analytical tools such as nuclear magnetic resonance (NMR), infrared (IR) and efficient separation techniques such as high-performance liquid chromatography (HPLC). Defining the structure of HA depended, to a large extent, on the data and information from the chemical behavior of HA and the chemical synthesis of HA from structurally similar compounds.
