ABSTRACT
Transformation systems based on mui-irzei-a --mes transposab?e elements have recently been shown to integrate heterologous genes into edes aegypti genome in a predictable manner (Coates et al.. 1998; Jasinskiene et al., 1998). This is an important accoinpPishment that should greatly facilitate gene manipu eion in mosquitoes. However, transforination remains an intensely laborious and time-consuming ocedure for characterization, mutagenesis, and expression of genes in mosquitoes. The propagation and maintenance of nnultiple transformed mosquito lines will be difficult in even the most spacious. well-equipped insectaries. Additionally, the comp?ex life cycle of some medically important mosquitoes make routine transgenesis difficult, whereas other transient expression systems may more easily and rapidly answer biological quesf o posed by researchers.
