ABSTRACT

The investigator who sets out to make a transgenic organism must consid~r two issues. First, a method of transformation must be chosen, or invented; and second, the transgene construct should be designed so that the transgenic organisms are maximally useful. The relative import of these two issues depends largely on the ease of making the transformanis. If an easy. well-established transformation procedure is available, then most em hasis will fall on designing the construct so that f ie informative experiments can be performed with the transgenic organisms. On the other hand, if transformation is difficult, or has not yet been achieved, then the construct may be primarily designed merely to achieve transformation. This chapter will discuss how site-specific recombination may be employed to aid in both aspects of iransgenesis. The majority of this chapter discusses methods of site-specific recombination that have been used in the model insect Dm.rophilu nzeln-

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?logaster: however. relevant resolts from other systems will also be introduced. Applicaf ons of sitespecific recombination in gene targeting and transgene rnanipulaiion will be considered. Finally. a

discovered nutation of D. inelnnognsrer will be considered as a poaentiai dominant marker gene For general insect transform.ation.