ABSTRACT
IE Medium Composition per 1011.0mL: Agar ..................................................................15.0g Peptone................................................................5.0g Yeast extract........................................................1.0g Basal salts solution............................................. 1.0L Lactose solution ................................................10.0g Trace elements solution .................................. 1.0mL
Basal Salts Solution: Composition per liter: MgSO4 ................................................................0.5g Phosphate solution ........................................ 20.0mL (NH4)SO4 (36% solution) ............................... 5.0mL
Phosphate Solution: Composition per liter: K2HPO4.............................................................95.0g NaH2PO4·2H2O.................................................78.0g
Preparation of Phosphate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
