ABSTRACT
Pancreatic digest of casein................................10.0g Glucose .............................................................10.0g Citric acid·H2O .................................................4.04g KH2PO4...............................................................2.0g Yeast extract........................................................1.5g CaCl2·2H2O.........................................................0.6g MgSO4·7H2O ......................................................0.6g FeCl2·4H2O .......................................................0.06g ZnSO4·7H2O ...................................................0.034g Hemin solution................................................ 1.0mL
pH 4.6 ± 0.2 at 25°C
Hemin Solution: Composition per 100.0mL: NaOH..................................................................1.0g Hemin...........................................................250.0mg
Preparation of Hemin Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121°C.
