ABSTRACT

OA-LUP See: Oat Meal Agar with Lupine Stems

Composition per liter: Oat flakes ..........................................................30.0g Agar ..................................................................15.0g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add rolled oats to 500.0mL distilled/deionized water. Gently heat and bring to boiling. Boil for 10 min. Filter. Add agar and bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Gently heat and bring to boiling. Mix by shaking. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure-121°C. Mix by shaking. Pour into sterile Petri dishes or leave in tubes.