ABSTRACT
Ham’s F-10 medium ..................................... 90.0mL Fetal bovine serum........................................ 10.0mL Penicillin-streptomycin solution ..................... 1.0mL
pH 7.0 ± 0.2 at 25°C
Ham’s F-10 Medium: Composition per liter: NaCl ....................................................................7.4g NaHCO3..............................................................1.2g Glucose ...............................................................1.1g NaH2PO4·H2O...................................................0.29g KCl....................................................................0.28g L-Arginine·HCl..................................................0.21g L-Glutamine.......................................................0.15g MgSO4·7H2O ....................................................0.15g Sodium pyruvate ...............................................0.11g KH2PO4.............................................................0.08g CaCl2·2H2O.......................................................0.04g L-Cystine·2HCl..................................................0.04g L-Histidine·HCl·H2O.........................................0.02g L-Lysine·HCl .....................................................0.02g L-Asparagine-H2O.............................................0.01g L-Aspartic Acid .................................................0.01g L-Glutamic acid .................................................0.01g L-Leucine...........................................................0.01g L-Proline ............................................................0.01g L-Serine .............................................................0.01g L-Alanine..........................................................8.9mg Glycine.............................................................7.5mg D-Phenylalanine ...............................................5.0mg L-Methionine ....................................................4.5mg Hypoxanthine...................................................4.1mg L-Threonine ......................................................3.6mg L-Valine ........................................................... 3.5mg L-Isoleucine ......................................................2.6mg L-Tyrosine.........................................................1.8mg Vitamin B12 ......................................................1.4mg Folic acid..........................................................1.3mg Phenol Red.......................................................1.2mg Thiamine·HCl ..................................................1.0mg FeSO4·7H2O.....................................................0.8mg Choline chloride...............................................0.7mg D-Calcium pantothenate ...................................0.7mg Thymidine........................................................0.7mg Niacinamide .....................................................0.6mg L-Tryptophan ....................................................0.6mg Isoinositol.........................................................0.5mg Riboflavin ........................................................0.4mg Lipoic acid .......................................................0.2mg Pyridoxine·HCl ................................................0.2mg ZnSO4·7H2O ..................................................0.03mg
volume to 1.0L. Mix thoroughly.
