ABSTRACT

Agar ..................................................................20.0g K2HPO4...............................................................7.0g KH2PO4...............................................................3.0g Glucose ...............................................................2.0g (NH4)2SO4...........................................................1.0g MgSO4·7H2O ......................................................0.1g CaCl2·2H2O.......................................................0.01g Indole ................................................................0.01g FeSO4·7H2O.....................................................0.5mg

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121°C. Pour into sterile Petri dishes or leave in tubes.