ABSTRACT

Agar ..................................................................15.0g Pancreatic digest of casein..................................3.0g CaCl2·2H2O.......................................................1.36g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH t o 7.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121°C. Pour into sterile Petri dishes or leave in tubes.