ABSTRACT

NaCl ................................................................116.9g Agar ..................................................................15.0g Tris-HCl ..........................................................6.024g NaHCO3............................................................1.68g MgSO4·7H2O ..................................................1.232g KNO3 ..............................................................0.505g CaCl2...............................................................0.033g KH2PO4...........................................................0.014g H3BO3 ..............................................................6.0mg MnCl2·4H2O .................................................. 99.0µg ZnCl2.............................................................. 14.0µg CoCl2·6H2O ................................................... 4.76µg CuCl2·2H2O ....................................................34.0ng FeCl3 solution ............................................... 50.0mL

pH 7.5 ± 0.2 at 25°C

FeCl3 Solution: Composition per 50.0mL: EDTA .............................................................5.84mg FeCl3 ..............................................................0.32mg

Preparation of FeCl3 Solution: Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly.