ABSTRACT
Composition per liter: Yeast extract......................................................30.0g Agar ..................................................................15.0g NaCl ..................................................................15.0g Pancreatic digest of gelatin ...............................10.0g Esculin ................................................................1.0g Nalidixic acid....................................................0.25g NaN3 .................................................................0.15g Cycloheximide ..................................................0.05g TTC solution ................................................. 15.0mL
pH 7.1 ± 0.2 at 25°C
TTC Solution: Composition per 15.0mL: 2,3,5-Triphenyltetrazolium chloride .................0.15g
Preparation of TTC Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 15.0mL. Mix thoroughly. Filter sterilize.
